Spinach carbonic anhydrase primary structure deduced from the sequence of a cDNA clone.
نویسندگان
چکیده
منابع مشابه
Carboxyl-terminal sequence of entactin deduced from a cDNA clone.
Entactin is a widely distributed basement membrane sulfated glycoprotein of approximately equal to 150 kDa. The entactin gene is expressed early in mouse embryogenesis. Two cDNA clones complementary to rat entactin mRNA were isolated by antibody screening of an oligo(dT)-primed cDNA library constructed in the lambda gt11 expression vector. One of the clones, lambda 1E, was subcloned into plasmi...
متن کاملSequence of a cDNA encoding carbonic anhydrase from barley.
CA catalyzes the reversible hydration of CO, to generate bicarbonate and a proton. Although its physiological function has not been clearly defined, it had been speculated that CA serves to concentrate CO, at the active site of Rubisco (Graham et al., 1984). However, recent work with transgenic plants expressing CA in the antisense orientation has shown that the enzyme does not play an essentia...
متن کاملNucleotide sequence of a cDNA encoding rice chloroplastic carbonic anhydrase.
CA (EC 4.2.1.1) catalyzes the reversible hydration of CO, according to the reaction CO, + H,O HCO, + H+ and represents 1 to 2% of total leaf soluble protein (Okabe et al., 1984). Considerable differences have been reported to exist between the molecular mass of the holoenzyme in monocotyledonous and dicotyledonous species (4245 kD in monocotyledonous plants and between 140 and 250 kD in dicotyl...
متن کاملPrimary structure of human carbonic anhydrase C.
The primary structure of human erythrocyte carbonic anhydrase C has been determined. The single polypeptide chain contains 259 amino acid residues devoid of disulfide bridges. The experimental approach has involved restriction of the action of trypsin to arginyl bonds by amidination of the lysyl side chains. The six tryptic fragments obtained have been separated and sequenced by manual techniqu...
متن کاملPrimary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence.
DNA sequences complementary to the double-stranded RNA coding for the neutralization antigen (glycoprotein VP7) of simian rotavirus SA11 have been cloned. The VP7 gene consists of 1,062 nucleotides, containing an uninterrupted coding sequence of 978 nucleotides which specifies a glycoprotein of 326 amino acids. The significance of a second possible initiation site 30 nucleotides downstream from...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1990
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)39375-5